Idaho Technology Inc. - 1-2-3 Sample Purification Kits

BioDefense
	- R.A.P.I.D.
	- RAZOR EX
	- JBAIDS
	- FilmArray
	- Reagents
	- Reagent Pouches
	- Sample Purification
	- Ready Response Kit
	- Training
Food Security Testing
Water Security Testing
Mutation Discovery
Genotyping

IT 1-2-3™ Sample Purification Kits

Our DNA/RNA extraction and purification kits enable extraction of DNA or RNA from pathogens found in food, water, blood and other sources. Each kit contains all of the necessary ingredients for DNA/RNA purification in a simple, complete and convenient format.

All kits contain easy to follow protocols requiring minimal training to reduce the risk of operator error. They have been designed to purify samples for analysis with Idaho Technology’s R.A.P.I.D.®, R.A.P.I.D. LT, and JBAIDS detection systems.

Purifying DNA or RNA

These kits allow you to purify bacteria and viruses from the same sample and include beads and reagent for mechanical lysis of spores. Each kit contains enough material to purify up to forty or fifty samples and do not require time-consuming enrichment steps. There are seven unique kits to extract DNA/RNA from bacteria and viruses from seventeen sample types. The protocols in this kit employ the steps described below.

DNA/RNA is extracted from the sample (e.g. cells or spores) through lysis. This is achieved by physical agitation and chemical disruption of the cells with bead-beating.
DNA/RNA is bound and concentrated on a filter.
The DNA/RNA on the filter is washed to remove inhibitors
The DNA/RNA is eluted from the filter.

	Download IT 1-2-3 Sample Purification Kit MSDS Sheets, Technical Notes, References, Manuals, and other Support Items
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	IT 1-2-3 Sample Purification Kit System Support

Purifying RNA

Purifying DNA

Sample Purification Kit Details

The following targets are available for the listed markets
Hazmat	Anthrax, Ricin, Smallpox, Plague
Food Safety	Listeria, E. coli 0157, Salmonella
Water Safety	Cryptosporidium, Salmonella
JBAIDS/US Federal	Tularemia, Ebola, Brucella, Anthrax IVD
Environmental	Avian Influenza, Botulism, Marburg

Our purification kits can purify the following samples
Medical	Blood, nasal, culture, sputum, gastric, lymph node, pus, stool
Food Safety	Milk, salad, ground beef, tuna
Hazmat	Air in PBS, surface, powder
Environmental	Air in PBS, soil, water, stool

Ordering Information
Kit Name	Description	Catalog No.
IT 1-2-3 SWIPE Sample Purification Kit*	Nasal swab DNA, surface swab DNA and RNA, live culture DNA and RNA, and powder DNA and RNA, pus swabs and lymph node aspirates samples	ASAY-ASY-0005
IT 1-2-3 FLOW Sample Purification Kit*†	Whole blood DNA, air in PBS DNA and RNA, water, milk, salad, ground beef, tuna salad, and gastric washing samples	ASAY-ASY-0004
IT 1-2-3 VIBE Sample Purification Kit*	Whole blood RNA, nasal swabs RNA, and sputum DNA and RNA	ASAY-ASY-0500
IT 1-2-3 SCOOP Sample Purification Kit	Stool and soil samples	ASAY-ASY-0502
IT 1-2-3 QFLOW DNA Sample Purification Kit*	Whole blood DNA, air in PBS DNA, water, milk, salad, ground beef, tuna salad and gastric washing samples	ASAY-ASY-0503
IT 1-2-3 QFLOW RNA Sample Purification Kit	Air in PBS RNA samples	ASAY-ASY-0504
IT 1-2-3 RNA Module	An accessory to the SWIPE, FLOW, VIBE and QFLOW DNA purification kits and contains items for the purification of RNA 1-2-3 FLOW samples	ASAY-ASY-0501
IT 1-2-3 DNA Sample Purification Kit	Sample purification and extraction kit for minimally trained technicians to extract DNA from environmental sources	3800

*These purification kits require the RNA Module (ASAY-ASY-0501) for some preparation applications.
†Large bead tube adapter (P/N PREP-ASY-0001) is required for this kit, but not included.

The IT 1-2-3 Sample Purification Kits are defense articles controlled for export under the International Traffic in Arms Regulations (ITAR), administered by the U.S. Department of State, Directorate of Defense Trade Controls. The transfer of any hardware or technical data controlled by the ITAR to any unauthorized persons, requires prior written approval for from the U.S. Department of State.