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Genotyping
FilmArray
LightScanner® Assays for Hi-Res Melting®

The following assays have been designed and optimized for use with the LightScanner® System. If you are interested in obtaining a protocol for any of the assays listed below please select the assay(s) and fill in and submit the form. We will send you the information related to that assay.

Many of the SNP genotyping assays use an unlabeled probe, also known as a LunaProbes™, in combination with LCGreen® Plus Dye, to genotype samples.

Send us your assay! If you are interested in a specific gene or SNP, please let us know. We are constantly updating our list and would love to include your gene of choice. Click here to submit your gene.
Step 1
 Click on the type of assay below to go to the specific list of scanning assays:
 

CANCER

CARDIAC

DRUG METABOLIZING ENZYMES - TRANSPORTERS

HEMOSTASIS

METABOLISM

NEURONAL
Step 2
After you select the desired assays, submit the form on the bottom of this page.

Select Assay
 Assay Gene Assay Type Mutation
METABOLISM
LPL LIPOPROTEIN LIPASE Whole gene analysis N/A
SCARB1 SCAVENGER RECEPTOR CLASS B, MEMBER 1 Whole gene analysis N/A
MCAD ACYL-CoA DEHYDROGENASE, MEDIUM-CHAIN LunaProbes Genotyping Assay 985 A>G
MCAD ACYL-CoA DEHYDROGENASE, MEDIUM-CHAIN LunaProbes Genotyping Assay 199 T>C
MCAD ACYL-CoA DEHYDROGENASE, MEDIUM-CHAIN Whole gene analysis N/A
OTC ORNITHINE CARBAMOYLTRANSFERASE Whole gene analysis N/A
NAGS N-ACETYLGLUTAMATE SYNTHASE Whole gene analysis N/A
GALT GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE Whole gene analysis N/A
MTHFR 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE LunaProbes Genotyping Assay A222V (677C>T)
MTHFR 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE LunaProbes Genotyping Assay E429A (1298A>C)
HFE HEMOCHROMATOSIS LunaProbes Genotyping Assay H63D (187C>G)
HFE HEMOCHROMATOSIS LunaProbes Genotyping Assay C282Y (845G>A)

Select Assay
 Assay Gene Assay Type Mutation
HEMOSTASIS
A1AT ALPHA-1-ANTITRYPSIN PROTEASE INHIBITOR Whole gene analysis N/A
Factor V COAGULATION FACTOR V LunaProbes Genotyping Assay R506Q (1691G>A)
Prothrombin COAGULATION FACTOR II LunaProbes Genotyping Assay 20210 G>A
CFHx9 COMPLEMENT FACTOR H Scanning T/C Y402H, T1277C poly

Select Assay
 Assay Gene Assay Type Mutation
NEURONAL
SOD1x1 SUPEROXIDE DISMUTASE 1 Inquire N/A
HTR2A SEROTONIN RECEPTOR Scanning x1 102 T>C
BMPR2 BONE MORPHOGENETIC PROTEIN RECEPTOR, TYPE II Whole gene analysis N/A

Select Assay
 Assay Gene Assay Type Mutation
CARDIAC
MYH7x3 MYOSIN, HEAVY CHAIN 7, CARDIAC MUSCLE, BETA Scanning N/A
MYH7x12 MYOSIN, HEAVY CHAIN 7, CARDIAC MUSCLE, BETA Scanning N/A
MYBPC3x3 MYOSIN-BINDING PROTEIN C, CARDIAC Scanning N/A
MYBPC3x4 MYOSIN-BINDING PROTEIN C, CARDIAC Scanning N/A
MYBPC3x6 MYOSIN-BINDING PROTEIN C, CARDIAC Scanning N/A
TNNT2x3,4 TROPONIN T2, CARDIAC (thin filaments, single fragment) Scanning N/A
TNNI3x7 TROPONIN I, CARDIAC (thin filaments) Scanning N/A
TNNI3x8 TROPONIN I, CARDIAC (thin filaments) Scanning N/A

Select Assay
 Assay Gene Assay Type Mutation
DRUG METABOLIZING ENZYMES/TRANSPORTERS
CYP1B1x2 Cytochrome P450, family 1, subfamily B, polypeptide 1 Scanning N/A
CYP1B1x3 Cytochrome P450, family 1, subfamily B, polypeptide 1 Scanning N/A
CYP2D6*4A Cytochrome P450, family 2, subfamily D, polypeptide 6 LunaProbes Genotyping Assay 1846 G>A
CYP2D6*2xN/
*35xN 		Cytochrome P450, family 2, subfamily D, polypeptide 6 LunaProbes Genotyping Assay 2850 C>T
CYP2C19*2 Cytochrome P450, family 2, subfamily C, polypeptide 19 LunaProbes Genotyping Assay +19154 G>A
CYP2C19*3 Cytochrome P450, family 2, subfamily C, polypeptide 19 LunaProbes Genotyping Assay +17948 G>A
CFTRx10 CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (ABCC7) Scanning F508del, F508C, I507del, I506V
CFTRx10 CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (ABCC7) LunaProbes Genotyping Assay F508del, F508C, I507del, I506V
CFTRx11 CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (ABCC7) LunaProbes Genotyping Assay G542X
ABCC2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2 LunaProbes Genotyping Assay C1515Y
(4544 G>A)

Select Assay
 Assay Gene Assay Type Mutation
CANCER
AXLx5 AXL RECEPTOR TYROSINE KINASE Scanning N/A
BRIP1 BRCA1-INTERACTING PROTEIN 1 Scanning N/A
BRAFx11 V-RAF MURINE SARCOMA VIRAL ONCOGENE HOMOLOG B1 Scanning N/A
BRAFx15 V-RAF MURINE SARCOMA VIRAL ONCOGENE HOMOLOG B1 Scanning N/A
EGFRx18 EPIDERMAL GROWTH FACTOR RECEPTOR Scanning N/A
EGFRx19 EPIDERMAL GROWTH FACTOR RECEPTOR Scanning N/A
EGFRx20 EPIDERMAL GROWTH FACTOR RECEPTOR
(Takes 3-4 weeks for delivery)		 Scanning N/A
EGFRx20 EPIDERMAL GROWTH FACTOR RECEPTOR
(Takes 3-4 weeks for delivery)		 LunaProbes Genotyping Assay 2361 G>A
EGFRx21 EPIDERMAL GROWTH FACTOR RECEPTOR Scanning N/A
ERBB2x23 V-ERB-B2 AVIAN ERYTHROBLASTIC LEUKEMIA VIRAL ONCOGENE HOMOLOG 2 Scanning N/A
ERBB2x24 V-ERB-B2 AVIAN ERYTHROBLASTIC LEUKEMIA VIRAL ONCOGENE HOMOLOG 2 Scanning N/A
FGF3Rx6 FIBROBLAST GROWTH FACTOR RECEPTOR 3 Scanning N/A
FGF3Rx8 FIBROBLAST GROWTH FACTOR RECEPTOR 3 Scanning N/A
FGF3Rx13 FIBROBLAST GROWTH FACTOR RECEPTOR 3 Scanning N/A
FGF3Rx17 FIBROBLAST GROWTH FACTOR RECEPTOR 3 Scanning N/A
JAK1x13 JANUS KINASE 1 Scanning N/A
JAK2x14 JANUS KINASE 2 Scanning N/A
JAK2x22 JANUS KINASE 2 Scanning N/A
KITx2 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx8 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx9 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx11 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx12/13 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx15 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
KITx17 V-KIT HARDY-ZUCKERMAN 4 FELINE SARCOMA VIRAL ONCOGENE HOMOLOG Scanning N/A
MSH2 MutL, E. COLI, HOMOLOG OF, 2 Whole gene analysis N/A
MLH1 MutL, E. COLI, HOMOLOG OF, 1 Whole gene analysis N/A
METx14 MET PROTOONCOGENE Scanning N/A
METx16 MET PROTOONCOGENE Scanning N/A
METx17 MET PROTOONCOGENE Scanning N/A
METx18 MET PROTOONCOGENE Scanning N/A
METx19 MET PROTOONCOGENE Scanning N/A
PDGFRAx10 PLATELET-DERIVED GROWTH FACTOR RECEPTOR, ALPHA Scanning N/A
PDGFRAx12 PLATELET-DERIVED GROWTH FACTOR RECEPTOR, ALPHA Scanning N/A
PDGFRAx18 PLATELET-DERIVED GROWTH FACTOR RECEPTOR, ALPHA Scanning N/A
PDPK1x3 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x4 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x5 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x6 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x7 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x8 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x9 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x10 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x11 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x12 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x13 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PDPK1x14 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Scanning N/A
PIK3Cax2 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax3 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax5 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax8 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax10 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax14 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax19 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
PIK3Cax21 PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, ALPHA Scanning N/A
ROS1 V-ROS AVIAN UR2 SARCOMA VIRUS ONCOGENE HOMOLOG 1 Scanning N/A
RETx10 REARRANGED DURING TRANSFECTION PROTOONCOGENE Scanning N/A
RETx16 REARRANGED DURING TRANSFECTION PROTOONCOGENE Scanning N/A
K-Rasx2 V-KI-RAS KIRSTEN RAT SARCOMA VIRAL ONCOGENE Scanning N/A
K-Rasx3 V-KI-RAS KIRSTEN RAT SARCOMA VIRAL ONCOGENE Scanning N/A
TP53x5 TUMOR PROTEIN p53 Scanning N/A

Please submit the following form to receive the protocols for the selected scanning assays.
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If you encounter any problems with this process, please contact Idaho Technology's technical support representative at 1-800-735-6544 or click here.
These assays are provided for informational purposes only and may be covered by intellectual property of third parties. No license is expressed or implied by the provision of assay information. The assays listed here are not validated for human clinical / diagnostic use. The genes and technologies that are involved with the assays posted may be covered by 3rd party intellectual property. LightScanner Master Mix is sold for research use only.
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